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Eady growth and proliferation in the monolayer system. Cells were cultured and treated as previously described [32]. Primary astrocyte cultures were prepared from the cortex of 6-day-old Sprague-Dawley rat pups as previously described [24]. The purity of primary astrocyte cultures was assessed with the astrocyte-Wang et al. Journal of Neuroinflammation 2010, 7:84 http://www.jneuroinflammation.com/